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. 2016 Jan 8;7(7):7801–7815. doi: 10.18632/oncotarget.6848

Figure 1. Overexpression of PHLPP isoforms inhibit PI3K/Akt and MEK/ERK signaling.

Figure 1

A. Stable control, HA-PHLPP1β or HA-PHLPP2 overexpressing Panc-1 cells were generated using retrovirus-mediated infection. The cell lysates were prepared and analyzed for phosphorylation and total protein expression by immunoblotting. B. Relative phosphorylation for p-Akt, p-MEK, p-ERK were calculated and normalized to those of total Akt, MEK and ERK, respectively. The level in control cells was set to 1. Data represent the mean ± SEM (n = 3, * p<0.05 by two-sample t-tests).