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. 2016 Jan 8;7(7):7801–7815. doi: 10.18632/oncotarget.6848

Figure 2. Loss of PHLPP expression enhances PI3K/Akt and MEK/ERK signaling.

Figure 2

A. Stable control (sh-Con) and PHLPP knockdown (sh-PHLPP1 and sh-PHLPP2) ASPC-1 cells were generated using lentivirus-mediated RNAi. The cell lysates were analyzed for phosphorylation and total protein expression by immunoblotting. B. Relative phosphorylation for p-Akt, p-MEK, p-ERK were calculated and normalized to those of total Akt, MEK and ERK, respectively. The level in control cells was set to 1. Data represent the mean ± SEM (n = 3, * p<0.05 by two-sample t-tests).