Figure 5. PHLPP inhibits the invasive phenotype of pancreatic cancer cells grown in 3D culture.

A–B. Stable control and PHLPP overexpressing Panc-1 cells were seeded in Matrigel and allowed to grow for 7 days. On the 3^rd day (A) and the 7^th day (B) after seeding phase-contrast images were taken with the 10X objective on using an inverted Nikon microscope. The boxed regions were enlarged and shown next to the original images. Scale bars, 100 μm. C. The size of cell clusters at day 7 was measured and analyzed using Nikon Element AR software. Data represent mean ± SEM (n = 18 cells/line, * p<0.05 by two-sample t-tests).