Table 5. Sequence of primers and probes used in the study.
| Loci | Genes | Primers | Sequence | Amplicon |
|---|---|---|---|---|
| 7q21.12 |
ABCB1 NG_011513.1 |
Forward | 5′-ttcaggtcggaatggatctt-3′ | 118 bp |
| Reverse | 5′-gcaactatgtaaactatgaaaatgaaa-3′ | |||
| Probe | FAM 5′-accgcaatggaggagcaaagaa-3′BHQ1 | |||
| 6p21.32 |
ABCB3 (TAP2) NG_009793.3 |
Forward | 5′-tactaacttgcctgggtcacata-3′ | 92 bp |
| Reverse | 5′-gtcagggagtataggcaactctt-3′ | |||
| Probe | FAM 5′-agaggtggacttgcccagctttg-3′BHQ1 | |||
| 16p13.1 |
ABCC1 NG_028268.1 |
Forward | 5′-tctctctggaattactgcgga-3′ | 101 bp |
| Reverse | 5′-acaggcatggagtcagctcta-3′ | |||
| Probe | FAM 5′-ccccaagagctgtaagccaagtc-3′BHQ1 | |||
| 10q24 |
ABCC2 NG_011798.1 |
Forward | 5′-gccacaggtatgtaagaaggatt-3′ | 95 bp |
| Reverse | 5′-tggatactgagcagttcaggaa-3′ | |||
| Probe | FAM 5′-catgggtggaatggtaaatcaatatc-3′BHQ1 | |||
| 3q27 |
ABCC5 NC_000003.12 |
Forward | 5′-gtcccaaccaaatcagaggt-3′ | 108 bp |
| Reverse | 5′-cctggtgctatattgtcaagaca-3′ | |||
| Probe | FAM 5′-cctcagaagcacccatgttagaaca-3′BHQ1 | |||
| 21q22.3 | ABCG1 NC_000021.9 | Forward | 5′-gcctgggtgatgagaaataat-3′ | 109 bp |
| Reverse | 5′-gctgacctgtgcctgtaaaa-3′ | |||
| Probe | FAM 5′-acactgacccatgaagagaaagcagt-3′BHQ1 | |||
| 4q22.1 | ABCG2 NG_032067.2 | Forward | 5′-gagttggtttgtgcttgtgtt-3′ | 100 bp |
| Reverse | 5′-attccattttaagtcaggttctatt-3′ | |||
| Probe | FAM 5′-agggtaggcactgaatatactcaatga-3′BHQ1 | |||
| 16p11.2 | MVP NC_000016.10 | Forward | 5′-cctgaaacagcacaggactg-3′ | |
| Reverse | 5′-tggaagcacccgcaaccctaa-3′ | |||
| Probe | FAM 5′-agggtaggcactgaatatactcaatga-3′BHQ1 | |||
| 3q13.33 |
CASR NG_009058.1 |
Forward | 5′-ccacctccacaacagcct-3′ | 105 bp |
| Reverse | 5′-gctggaggaggcataactga-3′ | |||
| Probe | FAM 5′-ctcagcacctcttcactcactcact-3′BHQ1 |
NG – number according to NCBI Nucleotide Database (http://www.ncbi.nlm.nih.gov/nuccore); bp: base pair; Forward – forward primer; Reverse - reverse primer.