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. 2016 Jan 9;7(7):7856–7865. doi: 10.18632/oncotarget.6856

Figure 4. GPR171 triggers invasion and migration of lung cancer cells.

Figure 4

A. Invasion and migration assays of A549 cells expressing control or GPR171 shRNA. Top, invaded A549 cells expressing control or GPR171 shRNA were stained with crystal violet. Invasion was quantified by counting cells in five randomly selected regions. Bottom, migrated A549 cells expressing control or GPR171 shRNA were stained with crystal violet. Migration was quantified by counting cells in eight randomly selected regions. Results are presented as means ± SEM (error bars) (***P < 0.001, Student's t test). B. Invasion and migration assays of Calu-6 cells expressing control or GPR171 shRNA. Top, invaded Calu-6 cells expressing control or GPR171 shRNA were stained with crystal violet. Invasion was quantified by counting cells in five randomly selected regions. Bottom, migrated Calu-6 cells expressing control or GPR171 shRNA were stained with crystal violet. Migration was quantified by counting cells in five randomly selected regions. Results are presented as means ± SEM (error bars) (***P < 0.001, Student's t test).