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. 2016 Jan 9;7(7):7866–7884. doi: 10.18632/oncotarget.6872

Figure 9. Effects of the MDM2 inhibitors on p53 protein accumulation.

Figure 9

U87MG cells were treated with DMSO (control), or 1 μM EB148 or 1 μΜ EB54 for 16 h, followed by a wash-out period of an additional 16 h. At the end of the treatment, p53 protein levels were evaluated by western blotting analysis using GAPDH as the loading control. A. Representative western blots. B. Densitometric analysis of the immunoreactive bands performed using the ImageJ program. The data were expressed as the percentage relative to control, and they are the mean values ± SEM of three different experiments. The significance of the differences was evaluated using a one-way ANOVA with the Bonferroni post-test: *p<0.05, **p<0.01 vs. control.