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. 2016 Jan 9;7(7):7913–7924. doi: 10.18632/oncotarget.6861

Figure 4. Correlation between irradiation, PD-L1 in cancer cells, and the function of cytotoxic T cells.

Figure 4

Figure 4

The levels of PD-L1 were evaluated by a. Western blot analysis and b. FACS with PD-L1 antibody for human esophageal cancer cells at indicated time after 6Gy irradiation or 48h after RT with 0, 3, 6, 9Gy in vitro. Representative slides are shown. Each column is shown as the means of 3 separate experiments; bars, SD.*, P<0.05. c. The effect of PD-L1 blockade on the suppressing ability of tumor cells for T cells proliferation was evaluated by FACS. Representative images and quantitative data are shown. Each column is shown as the means of 3 separate experiments; bars, SD.*, P<0.05; d. The effect of PD-L1 blockade on the CD8+ T cells cytotoxicity against cancer cells was evaluated by FACS. Representative images and quantitative data are shown.