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. 2016 Jan 12;7(7):7979–7992. doi: 10.18632/oncotarget.6888

Figure 7. SSA regulates miR-21 expression through the blockade of TGFβ signaling.

Figure 7

a. TGFβ1 at 30 ng/ml can induce miR-21 but not miR-10b, 17, and -9 expressions in MCF-7 cells as measured by qRT-PCR. b. SSA at 4 μM can inhibit the cell invasion induced by miR-21 mimics in MDA-MB-231 cells. BD Matrigel invasion assay was used to quantitate the motility of MDA-MB-231 cells in which the vehicle control or miR-21 mimics were transiently transfected. The transfected cells were also treated with SSA at 4 μM to observe its inhibitory effect on the invasive phenotype induced by miR-21. c. The combinations of SSA and TGFβ1 at different concentrations can alter the expression of miR-21 variably in MCF-7 cells as evaluated by qRT-PCR. d. The combinations of SSA and TGFβ1 at different concentrations can alter the expression of miR-21 variably in MDA-MB-231 cells as evaluated by qRT-PCR. *P < 0.05.