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Upper panels: SSA (4 μM) and SB431542 (10 μM) can attenuate the inductive effect of TGFβ1 (30 ng/ml) on phosphorylation of Smad-2/3 in MCF-7 and MDA-MB-231 cells as determined by Western blotting. Bottom panels: Blockade of TGFβ signaling by SSA or SB431542 can lead to downregulation of miR-21 in MCF-7 and MDA-MB-231 cells as determined by qRT-PCR. *P < 0.05.
