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. 2016 Jan 23;7(7):8119–8130. doi: 10.18632/oncotarget.6992

Figure 4. Reduced H3K27me3 in the HOX gene cluster is associated with low EZH2 expression in MDS primary CD34+ cells.

Figure 4

(A) ChIP-on-ChIP analysis revealed widely reduced H3K27me3 levels in the HOXA cluster locus in MDS patients. Reduced H3K27me3 peaks were observed in RAEB-1/2, RCMD, and RA compared to the normal controls. (B), (C) and (D) Similarly, ChIP-on-ChIP analysis revealed widely reduced H3K27me3 levels in HOXB, HOXC, and HOXD cluster loci in MDS patients. (E) ChIP-qPCR analysis revealed decreases in H3K27me3 levels at the HOXA, HOXB, HOXC, and HOXD loci in MDS patients. (F) ChIP-qPCR showed that EZH2 knockdown reduced H3K27me3 at the HOXA, B, C, and D cluster promoters in SKM-1 cells. Statistical significance relative to the control group is indicated: *p < 0.05; **p < 0.01; ***p < 0.001 (two-tailed, Student's t-test). Error bars show SEM.