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. 2016 Jan 23;7(7):8119–8130. doi: 10.18632/oncotarget.6992

Figure 5. EZH2 knockdown eliminates H3K27me3 marks in the HOX gene cluster and increases HOX gene expression.

Figure 5

(A) GEM analysis of MDS patients revealed higher expression of several HOXB, HOXC, and HOXD genes in patients with low EZH2 expression. (B) QRT-PCR revealed increased HOX gene expression in patients with low EZH2 expression. (C) GEM analysis of the EZH2 knockdown cells revealed higher expression of HOXA and HOXB genes. (D) QRT-PCR confirmed that elevated HOX gene cluster expression was observed in SKM-1 cells with EZH2 knockdown. Statistical significance relative to the control group is indicated: *p < 0.05; **p < 0.01; ***p < 0.001 (two-tailed, Student's t-test). Error bars show SEM.