Figure 5. Effect of E₂ on tumor angiogenesis and intratumoral TAM densities in in vivo preclinical models of ovarian cancer.

A. Immunohistochemical analysis showed in both experimental models a significant higher MVD (Microvessel Density) in E₂-treated (***P<0.001, n=8 tumors/group) compared to Ovx females. Representative images for CD31 immunostaining of tumors from Ovx and Ovx+E₂ mice (magnification 40x). B. Representative pictures for immunohistochemical staining of Arginase I-positive macrophages (Arg1) in HEY xenograft from E₂-treated mice. Magnification 20x and 40x. Results obtained showed a significant increase of the ratio Arg1/CD11b in E₂-treated compared to untreated Ovx mice (**P<0.01, n=8 tumors/group).