Table 1. Oligonucleotide primer sequences for real-time PCR.
Gene | Forward Primers (5′→3′) | Reverse Primers (5′→3′) | Expected size (bp) |
---|---|---|---|
ERα | CCACCAACCAGTGCACCATT | GGTCTTTTCGTATCCCACCTTTC | 108 |
ERβ1 | GTCAGGCATGCGAGTAACAA | GGGAGCCCTCTTTGCTTTTA | 181 |
ERβ2 | AGGCATGCGAGGGCAGAA | GGCCACCGAGTTGATTAGAGG | 115 |
ERβ5 | GATGCTTTGGTTTGGGTGAT | CCTCCGTGGAGCACATAATC | 165 |
GPER | AAACTGCGGTCAGATGTGGCT | TGTGTGAGGAGTGCAAG | 117 |
PR | TCAGTGGGCAGATGCTGTATTT | GCCACATGGTAAGGCATAATGA | 96 |
AR | AAGACGCTTCTACCAGCTCACCAA | TCCCAGAAAGGATCTTGGGCACTT | 170 |
GAPDH | TCCCTGAGCTGAACGGGAAG | GGAGGAGTGGGTGTCGCTGT | 218 |