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. 2016 Jan 22;7(7):8223–8239. doi: 10.18632/oncotarget.6972

Figure 2. miR-200 family and miR-203 expression are correlated with EMT-induced phenotype in HNSCC.

Figure 2

A. Expression of E-cadherin, ZEB1, and ZEB2 was examined by RT-PCR in cells with epithelial phenotype (HaCaT, HSC2, and MSCC-1) and in EMT-induced cells (MSCC-inv1, HOC313, KOSCC25B, KOSCC33A, and SpSCC). GAPDH was used as a control. B. Expression of miR-200a, -200b, -200c, -141, and -203 was examined by real-time PCR in cells with epithelial phenotype (HaCaT, HSC2, and MSCC-1) and in EMT-induced cells (MSCC-inv1, HOC313, KOSCC25B, KOSCC33A, and SpSCC). Expression of these miRNAs in HNSCC cells was normalized by that in normal keratinocytes (HaCaT). The graph shows the relative quantity of miRNAs (miR-200a, -200b, -200c, -203, and -141). C. The heat map of the miRNA expression of the experiment described above is shown. D. NMuMG cells were treated with 10 ng/mL of TGF-β. The figure shows the cell shape at 0 (no treatment) and 48 h after TGF-β treatment. E. Expression of E-cadherin, SNAI1, SNAI2, ZEB1, and ZEB2 mRNA was examined by real-time PCR at 0, 12, 24, and 48 h (n = 3) after treatment with 10 ng/mL of TGF-β in NMuMG cells. The graph shows the expression of these mRNAs (mRNA/GAPDH). All results are presented as means ± SD. **P < 0.01, *P < 0.05. F. Expression of miR-203 was examined by real-time PCR at 0, 12, 24, and 48 h (n = 3) after treatment with 10 ng/mL of TGF-β in NMuMG cells. The graph shows the expression levels of miR-203 normalized by U6. The results are presented as means ± SD and *P < 0.05.