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. 2015 Dec 19;7(7):8389–8398. doi: 10.18632/oncotarget.6690

Figure 3. Characteristics of prostate cancer cells with increased migration potential.

Figure 3

A.CCL2 concentrations in supernatants of C4-2 prt and mig cells were measured using an ELISA kit. B. mRNA levels of AR expressed by C4-2 prt and mig cells were analyzed using qRT-PCR. C. mRNA levels of TGF-β1 in C4-2 prt and mig cells were analyzed using qRT-PCR. D. WB analyses of N-cadherin (mesenchymal marker), E-cadherin (epithelial marker), and AR expression by C4-2 mig cells were performed, and the results were compared with those for C4-2 prt cells (mig1 and mig2 were independently collected). E. These data indicate that CCL2 induced the migration of prostate cancer cells and the EMT in vitro in the presence or absence of the suppression of androgen/AR signaling and that prostate cancer cells with higher metastatic potential secreted more CCL2 (red arrow).