Figure 1.

Botulinum neurotoxin A (BoNT/A) 96 h immobilizing dose response curves for zebrafish. Fish were injected intracoelomically with 10 µL of 40, 20, 10, 5, or 2.5 pg/fish BoNT/A diluted in gelatin phosphate buffer, (n = 10 fish/treatment/replicate). The results of three replicates are shown. The curves indicate the calculated dose effect by logit analysis on each replicate. The actual percentage immobilized for each dosage in each replicate is indicated by the symbol corresponding to the calculated curve of the same color. Blue curve and diamonds, orange curve and squares and green curve and triangles represent data from replicates 1, 2 and 3 respectively.