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. 2016 May 12;8(5):147. doi: 10.3390/toxins8050147

Figure 4.

Figure 4

Reactive oxygen species (ROS) generation in WE-treated cells: (A) Jurkat exposed to increasing concentrations of WE for 1 h, 3 h or 6 h. Cells treated with H2O2 0.1 mM for 15 min represent the positive control (dotted line parallel to x-axis). (B) Cells were treated for 6 h with WE 0.8 mg/mL in the absence or presence of o-phenanthroline (o-Phe, 10 μM), rotenone (Rot, 2 μM) or N-acetylcysteine (NAC, 10 mM). Cells treated with H2O2 0.1 mM for 15 min represent the positive control. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control, and °°° p < 0.001 versus WE.