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Pasteurization slightly reduces the biological activity of SEE at low concentrations. Phosphate buffered saline (PBS) was spiked with increasing concentrations of SEE and pasteurized at 63 °C for 30 min, 72°C for 15 s, and 89°C for 1 s. Pasteurized, spiked PBS (15 μL) was added to the Jurkat reporter cells. After incubation for 5 h, the luciferase enzyme activity was determined. The photon emission was detected by luminometer. Error bars represent standard errors.
