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Antibody affinity influence assay sensitivity. Milk (a) or PBS (b) was spiked with increasing concentrations of SEE and incubated for 16 h with immunomagnetic beads that can remove interference from food matrix. The toxin was disassociated from beads and incubated for 5 h with the Jurkat reporter cells. The luciferase enzyme activity was determined. The photon emission was detected by luminometer. Error bars represent standard errors.
