Table 5.
A summary of inter-run repeatability (RSD *) and accuracy of fortified kidney tissues using HPLC †.
| Fortification Levels, µg/g | RSD *, % | Average Nominal Concentration ‡, µg/g | Accuracy §, % |
|---|---|---|---|
| 15.0 | 6.4 | 14.5 | 3.3 |
| 20.0 | 9.8 | 18.6 | 7.1 |
| 30.0 | 5.6 | 31.6 | 5.2 |
| 40.0 | 1.3 | 40.8 | 2.1 |
| 50.0 | 2.3 | 49.2 | 1.5 |
* Relative standard deviation. It is based on three analyses. † High performance liquid chromatography. ‡ Nominal concentration is back-calculated from the calibration curve using the response of peak area from HPLC analysis. The average nominal concentration is calculated based on three analyses for each fortification level. § Accuracy is calculated as (Average nominal concentration-fortification level)/fortification level × 100%. It indicates the closeness of the concentration quantified by the calibration curve to the fortification level.