Figure 2.

(A) Schematic diagram of MaYMV cDNA cloning strategy. The relative positions of primers and RT-PCR products are shown as arrows and line segments, respectively. Vsi designation corresponds to primers that were designed based on virus-derived siRNA sequences. The red line represents the size and position for initially validation of MaYMV by using primer pairs F1/R1; and (B) a schematic representation of the genome organization of MaYMV. The predicted frameshift heptanucleotide is marked with a filled triangle, and the “leaky” amber stop codon with an asterisk. RdRp, RNA-dependent RNA polymerase; CP, coat protein; MP, movement protein; RTD, readthrough domain.