Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 28;8(5):120. doi: 10.3390/v8050120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Agroinoculation infectivity assays using pCB301-MaYMV and N. benthamiana plants. (A) RT-PCR analysis of not inoculated (systemic) leaves infected by MaYMV. The numbers from 1 to 12 correspond to individual plants inoculated with pCB301-MaYMV infectious clone. “+” represents the positive control (using plasmid pCB301-MaYMV as a PCR template); “−“ represents the negative control (using the cDNA from mock-inoculated N. benthamiana as a PCR template); and (B) Northern blot analysis of MaYMV RNA obtained from pCB301-MaYMV-agroinoculated upper leaves of plants and the MaYMV CP ORF-derived cDNA probes. Equal loading of total RNA (20 μg) was validated by ethidium bromide-stained agarose gels as indicated at the bottom of the panel. Numbers 1–3 correspond to the replicates of the negative control; numbers 4–6 are the replicates of samples from plants agro-inoculated with pCB301-MaYMV.