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. 2016 May 10;8(5):129. doi: 10.3390/v8050129

Figure 2.

Figure 2

Replication of recombinant HBV vectors in Huh-7 cells. (A) Southern blot analysis of replication of 5c3c and wild type HBV constructs with deletions in C ORF. Intracellular capsid-associated HBV DNA was prepared from Huh-7 cells transfected with indicated plasmids. Viral DNA was also prepared from HBV-infected patient serum to demonstrate the position of mature full-length wild type rcDNA; (B,C) Southern blot analysis of replication of 5c3c (B) and wild type HBV (C) constructs with deletions in C ORF in the presence or absence of trans-complemented HBV core. Intracellular capsid-associated HBV DNA was prepared from Huh-7 cells transfected with indicated plasmids; (D) Deletion in C ORF of 5c3c abolished rcDNA formation. Total DNA was extracted from Huh-7 cells transfected with indicated plasmids and analyzed in Southern blot. Positions of mature rcDNA species are indicated. Transfection efficiency was normalized using relative luciferase activities expressed by co-transfected firefly luciferase reporter measured using Luciferase Assay System (Promega).