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. 2016 May 10;8(5):129. doi: 10.3390/v8050129

Figure 3.

Figure 3

Recombinant HBV vector 5dCG allows versatile choice of cargo genes. (A) Schematic representation of 5dCG vector and multiple cargo genes tested. Cargo genes are represented as open boxes approximately drawn to scale and the actual lengths indicated to the right. Wild type HBV polymerase ORF that overlaps with preC/C, preS1/preS2/S and X ORFs is also depicted. Asterisks denote premature termination codons in preC/C and S ORF. Triangles denote ATG to ACG mutation of preS1 start codon that does not alter polymerase amino acid sequence. Artificial Gtx IRES sequences are represented as a patterned box. CMV, cytomegalovirus promoter. P, HBV polymerase. TP, terminal protein domain. RT, reverse transcriptase domain; (B) Southern blot analysis of replication of 5dCG vector harboring different cargo genes. Intracellular capsid-associated HBV DNA was prepared from Huh-7 cells transfected with indicated plasmids. Viral DNA was also prepared from HBV-infected patient serum to demonstrate the position of mature full-length wild type rcDNA. Transfection efficiency was normalized using relative luciferase activities expressed by co-transfected firefly luciferase reporter measured using the Luciferase Assay System (Promega).