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. 2016 May 12;8(5):131. doi: 10.3390/v8050131

Figure 2.

Figure 2

Protein 2B of CVB3 induces autophagy. (A) HeLa cells were co-transfected with pEGFP-2B and pmCherry-LC3 for 48 h. The control cells were co-transfected with pEGFP-C1 and pmCherry-LC3. Cells were observed by confocal microscope (×600); (B) cells with LC3 dots were counted over the LC3-expressing cells. *** p < 0.01. Data were derived from 100 cells in each sample; (C) HeLa cells were transfected with pEGFP-2B for 42 h and treated with E64-D/PEPA (10 ng/mL) for 6 h. Enhanced green fluorescent protein (EGFP) and LC3 were determined by Western blotting; (D) the ratio of LC3-II/LC3-I was calculated according to the results of Western blots. n = 4. Experiment was repeated four times. Representative images were presented.