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. 2016 May 20;8(5):141. doi: 10.3390/v8050141

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Vertebrate Cell Infection Assay. Mosquito (C6/36) and vertebrate (BHK, DF-1, Vero) cell monolayers were inoculated with PLV or CORV at an MOI of 1, or mock-infected and fixed 3 days post infect (A) or 9 days post infection (B). IFA analysis was performed with anti-dsRNA mAb 3G1 to detect replicating virus via dsRNA products [12]. Cell nuclei were stained with Hoechst 33342. Images were taken at ×40 magnification.

Vertebrate Cell Infection Assay. Mosquito (C6/36) and vertebrate (BHK, DF-1, Vero) cell monolayers were inoculated with PLV or CORV at an MOI of 1, or mock-infected and fixed 3 days post infect (A) or 9 days post infection (B). IFA analysis was performed with anti-dsRNA mAb 3G1 to detect replicating virus via dsRNA products [12]. Cell nuclei were stained with Hoechst 33342. Images were taken at ×40 magnification.