Table 1.
Summary of mosquito pools that tested positive for PLV by RT-PCR screening.
| Pool ID | Mosquito Species | Date Collected | Initial Isolation | Re-Isolation | % Pairwise Identity to Prototype (nt/aa) e | ||
|---|---|---|---|---|---|---|---|
| CPE | CPE | CPE | CPE | ||||
| C6/36 a | Vero b | PS-EK b | C6/36 c | ||||
| Kununurra | |||||||
| K74511 | Culex pullus | April 2011 | + | − | − | + | 99.75/99.25 |
| K74544 | Culex pullus | April 2011 | + | − | − | + | ND |
| K74770 | Culex annulirostris | April 2011 | + | − | − | + | 99.75/99.25 |
| K74775 | Culex species | April 2011 | + | − | − | + | 99.75/99.25 |
| K75023 | Culex annulirostris | April 2011 | + | − | − | + | 99.75/99.25 |
| Parry’s Creek | |||||||
| K71497 | Culex annulirostris | March 2010 | − | − | − | + | 97.47/95.49 |
| K71516 | Culex annulirostris | March 2010 | − | − | − | − | 97.74/95.49 |
| K71520 | Culex annulirostris | March 2010 | − | − | − | − | 97.74/95.49 |
| K71551 | Culex annulirostris | March 2010 | − | − | − | + | 100/100 |
| K71558 | Culex annulirostris | March 2010 | − | − | − | − | 97.74/95.49 |
| K71435 | Culex annulirostris | March 2010 | − | − | − | + | 99.50/98.50 |
| K75533 | Mansonia uniformis | April 2011 | + | − | − | + | 99.75/99.25 |
| Billiluna | |||||||
| K75737 | Aedes normanensis | April 2011 | + | − | − | + | 99.75/99.25 |
| K75749 d | Culex annulirostris | April 2011 | + | + d | + d | + | ND |
a On the 2nd passage; b 3rd passage (after 2 on C6/36 cells); c On the 3rd passage; d = indicative of a co-infection; e identity calculated over 399 bp region of segment 9; (+) CPE positive; (−) CPE negative; ND = Not Done.