Figure 1.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses of the recombinant rabies virus (RABV) L protein: (a) The purified recombinant C-terminal His-tagged RABV L protein (0.7 μg) was analyzed by 7.5% SDS-PAGE followed by Coomassie Brilliant Blue staining. Lane M shows marker proteins with the indicated molecular masses. Open and closed arrowheads indicate minor proteins co-purified with the RABV L protein; (b) The recombinant vesicular stomatitis virus (VSV) and RABV L proteins (10 ng) were analyzed by Western blotting with anti-His antibody.