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The RABV L protein caps virus-specific mRNAs: (a) The recombinant RABV L protein (0.1 μg) was incubated with [α-32P]GDP and 5′-triphosphorylated oligo-RNAs with the indicated sequences; (b) Vaccinia virus capping enzyme (Vac CE) was incubated with [α-32P]GTP and the RNAs. Nuclease P1-digests of RNA products were analyzed as in Figure 2. Lane 1 indicates no enzyme. The positions of the origin (ori.) and cap structures (GpppA, GpppG) are shown.
