Fig. 1.

Immunocytochemical stainings against pAkt of 0, 1, 10 or 100 nM insulin-stimulated HEK 293 cells permeabilized with saponin. Four different antibodies against pAkt (#4060, #2965, #3787, #4058) were tested in three independent experiments, but none of the antibodies showed strong insulin-induced staining when saponin was used as the permeabilizing agent.