Immunocytochemical stainings against pMAPK of 0, 1, 10 or 100 nM insulin-stimulated HEK 293 cells permeabilized with Saponin. Out of the four different antibodies tested against pMAPK (#4370, #9101, #4376 and #4377), only #9101 and #4370 showed weak increase in signals upon stimulation with insulin compared to non-stimulated cells. The antibodies were tested in three independent experiments.