Fig. 3.

Immunocytochemical stainings against pAkt of 0, 1, 10 or 100 nM insulin-stimulated HEK 293 cells permeabilized with Triton X-100. Here, #4060 anti-pAkt antibody showed the most specific and strongest staining of pAkt compared with #2965, #3787 and #4058. The antibodies were tested in three independent experiments.