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Immunocytochemical stainings against pMAPK of 0, 1, 10 or 100 nM insulin-stimulated HEK 293 cells permeabilized with Triton X-100. Using #4370 or #9101, a clear signal was seen upon stimulation compared to control. This was not the case when using #4370 or #4377. The antibodies were tested in three independent experiments.
