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Immunocytochemical stainings against pAkt of 0, 1 or 10 nM BDNF-stimulated hippocampal neurons permeabilized with Triton X-100. The two antibodies against pAkt, #4060 and #2965, gave the best staining of HEK 293 cells and was therefore tested on hippocampal neurons. In three independent experiments, the #4060 antibody gave the strongest and most specific staining of hippocampal neurons.
