Table 1.
List of primary antibodies against pAkt and pMAPK used in this paper.
| Antibody | Host | Clonality | Immunogen | Dilution factor | Company | Catalog nr. | Average pAbmAbs rating [1], [2], [3], [4], [5] |
|---|---|---|---|---|---|---|---|
| Anti-phospho-Akt (Ser473) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues around Ser473 of human Akt | 1:200 | Cell Signaling | 4060 |  |
| Anti-phospho-Akt (Thr308) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt | 1:800 | Cell Signaling | 2965 |  |
| Anti-phospho-Akt (Ser473) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt | 1:200 | Cell Signaling | 4058 |  |
| Anti-phospho-Akt (Ser473) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt | 1:50 | Cell Signaling | 3783 |  |
| Anti-Akt | Rabbit | Polyclonal | Synthetic peptide corresponding to the carboxy-terminal sequence of mouse Akt | 1:1000 | Cell Signaling | 9271 |  |
| Anti-phospho-MAPK (p44/42) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase | 1:100 | Cell Signaling | 4376 |  |
| Anti-phospho-MAPK (p44/42) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase | 1:200 | Cell Signaling | 4377 | |
| Anti-phospho-MAPK (p44/42) | Rabbit | Polyclonal | Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase | 1:1000 | Cell Signaling | 9101 | |
| Anti-phospho-MAPK (p44/42) | Rabbit | Monoclonal | Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase | 1:200 | Cell Signaling | 4370 | |
| Anti-MAPK | Rabbit | Polyclonal | Synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase | 1:1000 | Cell Signaling | 4695 | |