Subject area |
Biology |
More specific subject area |
Proteomics, Protein–protein interactions, Arabidopsis thaliana, plant biology, circadian rhythms, photoperiodism, affinity purification, mass spectrometry |
Type of data |
Table, figure |
How data was acquired |
Mass Spectrometry (LTQ-Orbitrap Velos Pro)coupled with HPLC (U3000 RSLCnano HPLC)
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Data format |
Raw, processed |
Experimental factors |
10–12-day-old seedlings of Arabidopsis thaliana expressing bait proteins were grown under diurnal conditions (light: dark=12 h:12 h with constant temperature (22 °C) and sampled at ZT12 (12 h after light is on). |
Experimental features |
FLAG-His tandem purification using magnetic beads coupled with on-beads trypsin digestion. High-resolution mass spectrometer coupled to nanoHPLC for protein identification and quantification. |
Data source location |
Saint Louis, Missouri, USA |
Data accessibility |
Data are within this article and have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 athttp://www.ebi.ac.uk/pride/archive/projects/PXD002606or dataset identifier PRIDE: PXD003352 athttp://www.ebi.ac.uk/pride/archive/projects/PXD003352
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