Fig. 7.

Survival curves for lethally irradiated NSG mice after transplantation with different amounts of human HSPCs. CD34⁺ human hematopoietic progenitor cells were isolated from umbilical cord blood and incubated in the absence (no treatment, group 1) or presence of the combination of treprostinil and 30 μM forskolin (in vitro pretreatment, groups 2 and 3); subsequently, the cell suspension (2.5 × 10⁵, 2 × 10⁵, and 1.5 × 10⁵ cells per mouse in the (A), (B), and (C) panels, respectively) was administered by tail vein injection to lethally irradiated recipient NSG mice. Some mice, which received pretreated cells, were also treated in vivo with treprostinil (in vitro and in vivo treatment, group 3; 0.15 mg kg⁻¹ 8 h⁻¹, s.c. and for 10 days), whereas others received no further treatment (in vitro pretreatment – group 2). Each group was comprised of 5 animals. The tables summarize the comparisons of the individual Kaplan-Maier plots by log-rank test.