Fig. 7.

MCDmp-induced chemoprevention mechanism. (a) cDNA microarray analysis of the tumor-free liver tissues showed that numerous genes regulating glycolysis, tumorigenesis, and inflammation were downregulated in Group 3. (b) NF-κB and IL-6 expression was markedly decreased in Group 3 on real-time PCR. (c) H&E staining (×100, upper panel) of the tumor-free liver tissues obtained from Group 1 mice at 4 weeks after transfection shows a distorted lobular arrangement, but liver architecture is grossly preserved in Group 3. TRITC–phalloidin immunostaining (lower panel) shows severe dilatation and distortion of the bile canaliculi (arrows) in Group 1. However, the polyhedral architecture of the bile canaliculi is grossly preserved in Group 3. Scale bar=5 μm.