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. Author manuscript; available in PMC: 2016 May 30.
Published in final edited form as: Cancer Res. 2014 Apr 22;74(12):3317–3331. doi: 10.1158/0008-5472.CAN-14-0772-T

Figure 4.

Figure 4

Mutant IDH1 affects TCA metabolism in vivo. A and B, box–whisker plots showing [1-13C]glutamine enrichment in plasma (A) and tumor (B) from mice with xenografts derived from the indicated cell lines. Black dot, mean; center line, median; box, interquartile range; whiskers, maximum and minimum values. C, relative 2-HG levels from xenografts, normalized to HCT116 parental xenografts. D, percentage of M1 αKG derived from [1-13C]glutamine in tumor xenografts. E, nMols αKG/nMols citrate ratio from xenografts grown from the indicated HCT116 cells. F, glutamine anaplerosis, as determined by M1 labeling of αKG relative to M1 labeling of glutamine from xenografts grown from the indicated HCT116 cells.