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. Author manuscript; available in PMC: 2016 May 30.
Published in final edited form as: Biotechnol J. 2015 Sep 10;10(10):1600–1611. doi: 10.1002/biot.201400749

Figure 4.

Figure 4

Biosynthetic fluxes to glycans and nucleotides are similar in cultured hESCs. (A) Percentage of labeled serine and ribose in cells cultured for 4 h after passaging in the presence of [U-13C6]glucose (UGlc). (B) Percentage of labeled glycan moieties from biomass in cells treated as in (A). (C) Quantitation of biosynthetic flux to different metabolites calculated using MIDs and molar pool sizes. (D) Comparison of fluxes to ribose versus glycans demonstrates similar biosynthetic needs in hESCs. Error bars represent SD (A–D) for three replicates. *, p-value between 0.01 and 0.05; **, p-value between 0.001 and 0.01; ***, p-value < 0.001 by Student’s two-tailed t-test.