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Gel detection of the 8 RNA samples from two-step RT-qPCR of pan-flavivirus and pan-alphavirus reactions: lanes 1, 13 and 25: Molecular weight markers, lanes 2 – 9: Test samples, lanes 10 and 22: No template controls, lanes 11, 12, 23 and 24: positive controls, lanes 14-21: test samples
