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. 2016 May;78(2):215–227.

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This is an Open Access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view the details of this license, please visit (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Histological changes in submandibular glands in experimental groups (PAS-Alcian blue staining, bar: 100 μm). a, b, c, : 3, 5 and 7 days after duct ligation (DL), respectively. d, e, f : 3, 5 and 7 days after duct obstruction (DO), respectively. g, h, i : 3, 5 and 7 days after periductal avulsion (PA). Insets in a,d,g are high magnification of each picture showing cell and nuclear measurement(dot line arrowed solid line arrowed)

2a: Atrophic epithelial cell features of acini (arrowheads) and dilation of intercalated and striated ducts (arrows) were observed after 3 days of DL. 2h: Marked atrophy and degeneration of epithelial cells were seen in all lobules, and almost all ducts were greatly dilated. Epithelial cells in the intercalated ducts showed mitoses, and scattered acini (arrowhead) could be seen in lobules despite the dilation of ducts (arrow) at 5 days after DL. 2c: At 7 days after DL, epithelial cells of acini had disappeared, while all ducts were extremely dilated (arrows). 2d: Epithelial cells of acini showed slight atrophy (arrowheads). Epithelial cells of the ducts were flattened and the lumens expanded (arrows) after 3 days of duct obstruction (DO). 2e: After 5 days DO, epithelial cells of acini had recovered morphologically (arrowheads) and ducts were slightly dilated. 2f: At 7 days after DO, epithelial cells of acini had recovered normal morphological features despite edematous tissue. 2g: Periductal avulsion (PA) caused the atrophy of epithelial cells in acini and the size of acini in lobules to be irregular. 2h: Atrophic and vacuolized epithelial cell features of acini (arrowheads) were observed after 3 days of PA. 2h: At 5 days after PA, epithelial cells of acini showed atrophic features (arrowhead), but ducts showed less change (arrow) and the interlobular space was dilated. 2i: At 7 days after PA, atrophy of acini had progressed (arrowhead), and spaces had grown larger, reflecting edema around ducts (arrows).

Fig. 2 — Histological changes in submandibular glands in experimental groups (PAS-Alcian blue staining, bar: 100 μm). a, b, c, : 3, 5 and 7 days after duct ligation (DL), respectively. d, e, f : 3, 5 and 7 days after duct obstruction (DO), respectively. g, h, i : 3, 5 and 7 days after periductal avulsion (PA). Insets in a,d,g are high magnification of each picture showing cell and nuclear measurement(dot line arrowed solid line arrowed)

2a: Atrophic epithelial cell features of acini (arrowheads) and dilation of intercalated and striated ducts (arrows) were observed after 3 days of DL. 2h: Marked atrophy and degeneration of epithelial cells were seen in all lobules, and almost all ducts were greatly dilated. Epithelial cells in the intercalated ducts showed mitoses, and scattered acini (arrowhead) could be seen in lobules despite the dilation of ducts (arrow) at 5 days after DL. 2c: At 7 days after DL, epithelial cells of acini had disappeared, while all ducts were extremely dilated (arrows). 2d: Epithelial cells of acini showed slight atrophy (arrowheads). Epithelial cells of the ducts were flattened and the lumens expanded (arrows) after 3 days of duct obstruction (DO). 2e: After 5 days DO, epithelial cells of acini had recovered morphologically (arrowheads) and ducts were slightly dilated. 2f: At 7 days after DO, epithelial cells of acini had recovered normal morphological features despite edematous tissue. 2g: Periductal avulsion (PA) caused the atrophy of epithelial cells in acini and the size of acini in lobules to be irregular. 2h: Atrophic and vacuolized epithelial cell features of acini (arrowheads) were observed after 3 days of PA. 2h: At 5 days after PA, epithelial cells of acini showed atrophic features (arrowhead), but ducts showed less change (arrow) and the interlobular space was dilated. 2i: At 7 days after PA, atrophy of acini had progressed (arrowhead), and spaces had grown larger, reflecting edema around ducts (arrows).