Table 1.

Number of identified known miRNAs in P. tomentosa based on high-throughput sequencing.

Sample	Known miRNAs	miRNA*	miRNA* > miRNA	Unique miRNAs	Difference
D7	174	88	20	75	44
D10	175	98	21	78	48
D12	171	94	16	75	45
D16	178	100	20	77	47
D18	187	121	33	83	50
D21	193	106	27	79	44
Total	209	133	41	91	56
Consistent among six libraries	157	70	11	65	36

“Known miRNAs,” conserved miRNAs between P. tomentosa and P. trichocarpa; “Total,” number of reads identified in at least one library; “Consistent among six libraries,” number of reads identified during SVS regeneration in all six small RNA libraries; “miRNA^* ≥ miRNA,” the number of miRNAs^* matched to an equivalent or greater number of sequences than the corresponding miRNA. “Unique miRNAs,” the number of members of a given miRNA family with identical sequences; “Difference,” the number of instances in which the unique miRNA sequence with the most reads was not the mature miRNA in miRBase 18.0.