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. 2016 May 31;4:52. doi: 10.3389/fcell.2016.00052

Table 1.

Averaged relative abundances of individual N-glycan structures isolated from Y101 hTERT-MSCs and their osteoblast progeny as well as MSCs kept for 21 days in basal medium.

Glycan composition Typea Average relative abundanceb
MSC Osteoblast 21 day basal
Hex5HexNAc2 O 2.394 ± 0.120 3.505 ± 1.182 7.095 ± 1.526
Fuc1Hex5HexNAc2 O 0.621 ± 0.215 0.187 ± 0.187 0.885 ± 0.405
Hex6HexNAc2 O 10.740 ± 0.998 6.846 ± 1.639 13.342 ± 2.521
Hex7HexNAc2 O 6.470 ± 0.480 3.010 ± 0.499 6.258 ± 1.252
Hex8HexNAc2 O 13.042 ± 0.894 3.476 ± 0.160 9.089 ± 0.790
Hex9HexNAc2 O 10.693 ± 0.825 2.383 ± 0.345 5.174 ± 0.521
Hex10HexNAc2 O 1.119 ± 0.154 0 0.186 ± 0.062
Hex5HexNAc3 H 0.584 ± 0.114 0.509 ± 0.328 0.615 ± 0.166
Hex6HexNAc3 H 0.212 ± 0.130 0.638 ± 0.370 0.916 ± 0.192
Hex6HexNAc4 H 0.965 ± 0.217 1.557 ± 0.483 1.246 ± 0.351
NeuAc1Hex6HexNAc3 H 0.432 ± 0.184 0.594 ± 0.302 0.709 ± 0.090
NeuAc1Fuc1Hex6HexNAc3 H 0.032 ± 0.032 0 0
Hex7HexNAc5 H 0.438 ± 0.127 0.207 ± 0.089 0.125 ± 0.040
NeuAc2Hex6HexNAc4 H 0.581 ± 0.108 1.032 ± 0.084 0.304 ± 0.269
Hex8HexNAc6 H 0.066 ± 0.031 0 0
Hex3HexNAc3 C 0.004 ± 0.004 0.178 ± 0.178 0.091 ± 0.112
Fuc1Hex3HexNAc3 C 0.431 ± 0.084 0.185 ± 0.185 0.292 ± 0.195
Hex4HexNAc3 C 0.335 ± 0.085 0.197 ± 0.197 0.323 ± 0.112
Fuc1Hex3HexNAc4 C 0.686 ± 0.106 0.126 ± 0.126 0.287 ± 0.101
Hex4HexNAc4 C 0.285 ± 0.056 0.089 ± 0.089 0.224 ± 0.134
Fuc1Hex4HexNAc4 C 0.552 ± 0.119 0 0
Hex5HexNAc4 C 2.151 ± 0.214 1.622 ± 0.485 3.370 ± 0.447
Fuc1Hex3HexNAc5 C 0.297 ± 0.093 0 0.000 ± 0.138
NeuAc1Fuc1Hex4HexNAc3 C 0.379 ± 0.104 0.139 ± 0.139 0.597 ± 0.269
Fuc1Hex5HexNAc4 C 8.259 ± 0.655 12.271 ± 1.386 11.951 ± 2.082
NeuAc1Fuc1Hex5HexNAc3 C 0 0.138 ± 0.138 0.337 ± 0.132
NeuAc1Hex5HexNAc4 C 3.040 ± 0.347 3.757 ± 0.367 3.330 ± 0.169
Hex6HexNAc5 C 0.383 ± 0.123 0.088 ± 0.088 0.204 ± 0.045
NeuAc1Fuc1Hex5HexNAc4 C 11.968 ± 0.722 25.387 ± 2.830 12.152 ± 4.007
NeuGc1Fuc1Hex5HexNAc4 C 1.348 ± 0.273 3.712 ± 0.423 1.346 ± 0.789
Fuc1Hex6HexNAc5 C 1.661 ± 0.213 1.171 ± 0.382 0.590 ± 0.184
NeuAc2Hex5HexNAc4 C 1.421 ± 0.340 1.808 ± 0.547 0.619 ± 0.078
NeuAc1Fuc1Hex5HexNAc5 C 0.143 ± 0.059 0 0
NeuAc1Hex6HexNAc5 C 0.461 ± 0.101 0.314 ± 0.150 0.125 ± 0.033
NeuGc1Hex6HexNAc5 C 0.252 ± 0.052 0.140 ± 0.112 0.040 ± 0.032
NeuAc2Fuc1Hex5HexNAc4 C 4.784 ± 0.583 7.695 ± 1.401 2.957 ± 1.398
NeuAc1Fuc1Hex6HexNAc5 C 2.102 ± 0.360 1.827 ± 0.904 0.599 ± 0.323
NeuGc1Fuc1Hex6HexNAc5 C 0.351 ± 0.086 0.241 ± 0.103 0.071 ± 0.044
Fuc1Hex7HexNAc6 C 0.398 ± 0.084 0.134 ± 0.082 0.043 ± 0.028
NeuAc2Hex6HexNAc5 C 0.248 ± 0.096 0.274 ± 0.050 0.249 ± 0.238
NeuAc1Fuc6Hex4HexNAc4 C 0.025 ± 0.016 0 0
NeuAc2Fuc3Hex5HexNAc4 C 0.011 ± 0.011 0 0
NeuAc1Hex7HexNAc6 C 0.091 ± 0.030 0 0
NeuAc2Fuc1Hex6HexNAc5 C 1.228 ± 0.269 1.670 ± 0.932 0.345 ± 0.328
NeuAc1NeuGc1Fuc1Hex6HexNAc5 C 0.126 ± 0.036 0.210 ± 0.097 0.037 ± 0.057
NeuAc1Fuc1Hex7HexNAc6 C 0.588 ± 0.147 0.321 ± 0.242 0.058 ± 0.049
NeuAc1Hex8HexNAc6 C 0.075 ± 0.029 0.042 ± 0.042 0.000 ± 0.013
NeuGc2Hex6HexNAc6 C 0.014 ± 0.014 0.058 ± 0.058 0
NeuAc3Hex6HexNAc5 C 0.657 ± 0.268 2.034 ± 1.445 0.027 ± 0.033
NeuAc2NeuGc1Hex6HexNAc5 C 0.045 ± 0.027 0.288 ± 0.194 0
NeuAc2Hex7HexNAc6 C 0.077 ± 0.041 0.092 ± 0.092 0
NeuAc1NeuGc1Hex7HexNAc6 C 0 0.054 ± 0.054 0
NeuAc3Fuc1Hex6HexNAc5 C 0.373 ± 0.114 0.993 ± 0.611 0.089 ± 0.135
NeuAc2NeuGc1Fuc1Hex6HexNAc5 C 0.036 ± 0.015 0.167 ± 0.100 0.014 ± 0.028
NeuAc2Fuc1Hex7HexNAc6 C 0.520 ± 0.176 0.735 ± 0.587 0.068 ± 0.104
NeuAc1NeuGc1Fuc1Hex7HexNAc6 C 0.038 ± 0.025 0.088 ± 0.076 0.009 ± 0.018
NeuAc1Fuc1Hex8HexNAc7 C 0.047 ± 0.030 0 0
NeuAc4Hex6HexNAc5 C 0.083 ± 0.043 0.377 ± 0.255 0
NeuAc1Fuc1Hex7HexNAc8orc
NeuAc3NeuGc1Hex6HexNAc5 		C 0 0.065 ± 0.040 0
NeuAc3Hex7HexNAc6 C 0.023 ± 0.014 0.037 ± 0.037 0
NeuAc2Hex8HexNAc7 C 0.004 ± 0.004 0.024 ± 0.024 0
NeuAc1NeuGc1Hex8HexNAc7 orc
NeuGc2Fuc1Hex7HexNAc7 		C 0 0.016 ± 0.016 0
NeuAc3Fuc1Hex7HexNAc6 C 0.174 ± 0.077 0.427 ± 0.332 0.024 ± 0.057
Fuc2Hex8HexNAc9 orc
NeuAc3Hex8HexNAc6 		C 0 0.057 ± 0.047 0.000 ± 0.009
NeuAc2Fuc1Hex8HexNAc7 C 0.033 ± 0.020 0.070 ± 0.060 0.000 ± 0.006
NeuAc4Fuc1Hex7HexNAc6 C 0.046 ± 0.024 0.233 ± 0.177 0.005 ± 0.017
NeuAc3Fuc1Hex8HexNAc7 C 0.016 ± 0.010 0.061 ± 0.051 0
NeuAc2Fuc1Hex9HexNAc8 C 0.008 ± 0.005 0.013 ± 0.013 0
NeuAc4Fuc1Hex8HexNAc7 C 0.002 ± 0.002 0.019 ± 0.014 0
NeuAc3Fuc1Hex9HexNAc8 C 0.001 ± 0.001 0.011 ± 0.011 0
Fuc1Hex2HexNAc2 DPd 0.745 ± 0.190 0.746 ± 0.221 1.955 ± 0.311
Hex3HexNAc2 DP 0.891 ± 0.220 1.053 ± 0.422 2.830 ± 0.603
Fuc1Hex3HexNAc2 DP 2.402 ± 0.504 3.291 ± 1.230 6.714 ± 1.163
Hex4HexNAc2 DP 0.618 ± 0.106 1.216 ± 0.282 1.641 ± 0.323
Fuc1Hex4HexNAc2 DP 0.396 ± 0.095 0 0.571 ± 0.227
a

Glycans grouped as oligomannose (O), hybrid (H), complex (C), and degradation product (DP).

b

Percentage of total glycan signal ± SEM for n = 5 (MSC), n = 3 (osteoblast) and n = 2 (21 day basal) are shown.

c

Given the mass of these glycans other glycan compositions are possible, but the most plausible two possibilities are shown.

d

DP are small glycans that are not produced by the mammalian N-glycosylation machinery and therefore most likely originate from lysosomal degradation.