Table 2.
Statistical analysis of neurogenesis measures.
| Immunos | N | Measurement | Statistical test | Factor | Statistic | p-value | Figures | |
|---|---|---|---|---|---|---|---|---|
| Ki-67 | 10–15 mice/group | Proliferation | 2-way ANOVA | Induction | F(1, 46) = 0.103 | 0.7492 | Figure 4B | |
| Treatment | F(1, 46) = 0.250 | 0.6198 | ||||||
| Interaction | F(1, 46) = 0.127 | 0.7231 | ||||||
| BrdU | 14–16 mice/group | Survival | 2-way ANOVA | Induction | F(1, 54) = 7.897 | 0.0069 | Figure 4C | |
| Treatment | F(1, 54) = 25.980 | <0.0001 | ||||||
| Interaction | F(1, 54) = 0.002 | 0.9615 | ||||||
| Planned Comparison t-test | CTRL mice | Treatment | t(26) = −4.472 | 0.0001 | ||||
| iGluN2BNes mice | Treatment | t(28) = −3.143 | 0.0039 | |||||
| BrdU/NeuN/GFAP | 3 mice/group 19–39 cells/mouse | %BrdU+/NeuN+ | 2-way ANOVA | Induction | F(1, 8) = 0.060 | 0.8133 | Figure 4D | |
| Treatment | F(1, 8) = 7.332 | 0.0268 | ||||||
| Interaction | F(1, 8) = 0.439 | 0.5261 | ||||||
| Planned Comparison t-test | CTRLmice | Treatment | t(4) = −0.870 | 0.4334 | ||||
| iGluN2BNes mice | Treatment | t(4) = −2.770 | 0.0503 | |||||
| %BrdU+/GFAP+ | 2-way ANOVA | Induction | F(1, 8) = 0.001 | 0.0775 | ||||
| Treatment | F(1, 8) = 4.715 | 0.0617 | ||||||
| Interaction | F(1, 8) = 0.971 | 0.3534 | ||||||
| Planned Comparison t-test | CTRLmice | Treatment | t(4) = 1.226 | 0.2874 | ||||
| iGluN2BNes mice | Treatment | t(4) = 2.292 | 0.0837 | |||||
| DCX | 8–16 mice/group | Immature Neuron Number | 2-way ANOVA | Induction | F(1, 41) = 0.125 | 0.7253 | Figure 4F | |
| Treatment | F(1, 41) = 6.851 | 0.0124 | ||||||
| Interaction | F(1, 41) = 0.578 | 0.4514 | ||||||
| Planned Comparison t-test | CTRLmice | Treatment | t(20) = −2.909 | 0.0087 | ||||
| iGluN2BNes mice | Treatment | t(21) = −1.141 | 0.2667 | |||||
| 6 mice/group | Tertiary Dendrites | 2-way ANOVA | Induction | F(1, 20) = 0.532 | 0.4742 | Figure 4G | ||
| Treatment | F(1, 20) = 6.356 | 0.0203 | ||||||
| Interaction | F(1, 20) = 0.511 | 0.4832 | ||||||
| Planned Comparison t-test | CTRLmice | Treatment | t(10) = −2.022 | 0.0708 | ||||
| iGluN2BNes mice | Treatment | t(10) = −1.506 | 0.1629 | |||||
| BrdU/DCX | 3 mice/group 2–5 cells/ mouse | Dendritic length | 2-way ANOVA | Induction | F(1, 34) = 1.724 | 0.1980 | Figure 4H | |
| Treatment | F(1, 34) = 2.222 | 0.1453 | ||||||
| Interaction | F(1, 34) = 0.995 | 0.3256 | ||||||
| Planned Comparison t-test | CTRLmice | Treatment | t(15) = −1.739 | 0.1025 | ||||
| iGluN2BNes mice | Treatment | t(19) = −0.358 | 0.7246 | |||||
| Sholl Analysis | Repeated measures ANOVA—FLX treated mice | Induction | F(1, 18) = 2.152 | 0.1596 | Figure 4I | |||
| Radius | F(20, 360) = 24.446 | <0.0001 | ||||||
| Radius × Induction | F(20, 360) = 1.174 | 0.2742 | ||||||
| t-test | radius 70 | Induction | t(18) = 3.760 | 0.0014 | ||||
| radius 80 | Induction | t(18) = 2.202 | 0.0410 | |||||
| Repeated measures ANOVA—VEH treated mice | Induction | F(1, 16) = 0.117 | 0.7363 | Figure 4J | ||||
| Radius | F(20, 320) = 16.139 | <0.0001 | ||||||
| Radius × Induction | F(20, 320) = 0.187 | >0.9999 | ||||||
p < 0.05 are highlighted in bold.