Table 1. Primer sequences used in real-time PCR.
| Gene name | Primer sequence (5′-3′) | Accession number |
|---|---|---|
| Oct-4 |
GGAGGAAGCTGACAACAATGAAA |
NM_001285987 |
| (POU5F1) |
TACAGAACCACACTCGGACCAC |
|
| NANOG |
GCAAGAACTCTCCAACATCCTGA |
NM_024865 |
| |
CCTTCTGCGTCACACCATTG |
|
| PAX6 |
GAATGGGCGGAGTTATGATACCTA GAAATGAGTCCTGTTGAAGTGGTG |
NM_001258465 |
| SOX2 |
ACACTCACGCAAAAACCGC AAAGCTCCTACCGTACCACTAGAACT |
NM_003106 |
| LHX2 |
CAACCCTCTGGGTCTTCCCT GTGCTCTGCGTCGTTTTCGT |
NM_004789 |
| RAX |
GCGTTCGAGAAGTCCCACTAC |
NM_013435 |
| |
GTCGGTTCTGGAACCACACC |
|
| CHX10 (VSX2) |
GCTACTGGGGATGCACAAAAA CGTCCTGCTCCATCTTGTCG |
NM_182894 |
| SIX3 |
GGGCAGAAAACATAAAAGAGGTGAC CCCAGATACAAATAAATCGTCATGC |
NM_005413 |
| SIX6 |
GCTGTTTATTTACTTATTTAAGAGACCGC TCTCCACACAGAACCCATCACC |
NM_007374 |
| MITF |
GAGAAGAAACTGGAGCACGCC AATCTGGAGAGCAGAGACCCG |
NM_198159 |
| ATOH7 |
CAGCCTGGTCATCCAGTAGAACA GAGCAAATAAGTCATAAACAAAGCAAC |
NM_145178 |
| BRN3B |
GCGCTCAAACCCATCCTG |
NM_004575 |
| (POU4F2) |
GGCTGAATGGCAAAGTAGGC |
|
| iSlet1 |
ACACCTTGGGCGGACCTGCTATG |
NM_002202 |
| |
TGAAACCACACTCGGATGACTCTG |
|
| HOXB4 |
CCGTCGTCTACCCCTGGATG |
NM_024015 |
| |
CCGTGTCAGGTAGCGGTTGTAG |
|
| AFP |
CAAAATGCGTTTCTCGTTGCTT |
NM_001134 |
| |
GTGTCCGATAATAATGTCAGCCG |
|
| ACTB | AAGATGACCCAGATCATGTTTGAG TGAGGTAGTCAGTCAGGTCCCG | NM_001101 |