Fig. 4.

Effect of Rsv on human TP53 promoter activity. (A–D) (Left panel) The 5’-flanking region of the human TP53 gene, which has been ligated upstream of the Luciferase gene of the pGL4.10[luc2], is shown. The 5’-end of the cDNA is designated +1. Putative transcription factor binding elements that were found from the TF-SEARCH program (score > 87) are schematically shown. The sequence of the TP53 promoter contained in each deletion construct is schematically indicated. (A) Luc reporter plasmids were transiently transfected into HeLa S3 cells and treated with (closed bars) or without (open bars) Rsv (20 µM) for 24 h, then they were collected after further 24 h incubation. Luc activities were normalized to that of the pGL4-PIF1-transfected cells. Histograms show relative Luc activities of deletion construct transfected cells comparing with that of the pGL4-TP53-551-transfected cells without Rsv treatment. (B–D) Similar experiments as described under (A) were carried out with pGL4-TP53-551, -ΔA, -ΔB, -ΔC, -ΔD, -ΔE, -ΔF, -ΔG, -ΔH, -ΔI, -WT, -mt1, -mt3, -mt13 and pGL4.10[luc2]. Results are shown as means ± SD from five independent experiments. Statistical analysis was performed with the Student’s t test and asterisks indicate values of P < 0.05.