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RT-PCR and FISH for detecting ALK rearrangement on primary tumor and paired metastatic lymph nodes in the same patients. (A,B) ALK positive on primary tumor by RT-PCR and FISH, respectively; (C,D) ALK negative on paired metastatic lymph node by RT-PCR and FISH in the same patient, respectively. RT-PCR, reverse transcriptase-polymerase chain reaction; FISH, fluorescence in situ hybridization.
