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. 2016 May 17;2016:4712053. doi: 10.1155/2016/4712053

Figure 1.

Figure 1

Construction of nanoparticles. (a) Titration of heparin-peptide complex with protamine. Protamine samples (60 μL) containing 15 μg heparin and 200 nM peptide in PBS were tested for the particle hydrodynamic radius by dynamic light scattering (DLS). The proportion of particles with a radius of ~100 nm is plotted. (b) Representative DLS profile; peptide-heparin-protamine core. Representative DLS profile of particles containing 0.3 : 1 protamine : heparin (w : w) together with 200 μM P2 (KYCCSRK). (c) Titration of core complex with membrane lipids. Core particles were prepared as in (b), and increasing amounts of membrane particles (Section 2) were added prior to measurement of hydrodynamic radius by DLS. P2D P2 peptide built of D-amino acids. (d) Representative DLS profile, fully assembled nanoparticles. Representative DLS profile of particles with core structures prepared as in (b), after addition of 0.4 mM membrane lipid.