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. 2016 May 20;4(10):e12791. doi: 10.14814/phy2.12791

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of CB on MHC and fiber‐type composition in TA and SOL muscles. Typical SDS‐PAGE profiles of MHC isoforms (upper) and the average MHC composition (lower) of TA (A) and SOL (B) prepared from Control and CB‐treated (CB) WT and Epac1KO. The average proportion of each MHC isoform was expressed as a percentage of total MHC content (% of total MHC). (A) The proportions of MHC‐IId/x and MHC‐IIb in TA muscle were not significantly different between WT and Epac1KO in either the Control or CB‐treated group (P = NS vs. WT by Tukey's test, n = 6). (B) The proportion of MHC‐I in SOL prepared from Epac1KO was significantly greater than that of WT in both the Control and CB‐treated groups (*P < 0.05, **P < 0.01 vs. WT by Tukey's test, n = 6), while that of MHC‐IIb in SOL prepared from Epac1KO was significantly smaller than that of WT in both the Control and CB‐treated groups (**P < 0.01 vs. WT by Tukey's test, n = 6). The proportions of MHC‐IIa and MHC‐IId/x were not significantly different between WT and Epac1KO in either the Control or CB‐treated group (P = NS vs. WT by Tukey's test, n = 6). (C) Representative cross sections of immunohistochemical staining for type I (upper) and type II (lower) fibres in SOL prepared from Control (left) and CB‐treated (CB) (right) WT and Epac1KO. (D) Quantitative comparison of fiber type composition in SOL between WT and Epac1KO in the Control and CB‐treated groups. The proportion of type I fiber in Epac1KO was significantly greater than that in WT (**P < 0.01 vs. WT by Tukey's test, n = 6), while that of type II fiber in Epac1KO was significantly smaller than that in WT in both the Control and CB‐treated groups (*P < 0.05, **P < 0.01 vs. WT by Tukey's test, n = 6). The proportion of intermediate type (Type I/II) was not significantly different between WT and Epac1KO in either the Control or CB‐treated groups (P = NS vs. Control by Tukey's test, n = 6). The average proportion of fiber type was expressed as a percentage of total fiber number (% of total fiber).